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Schuele Lab Tutorials, Videos, and Protocols

The Schuele lab at The Parkinson's Institute is actively engaged in basic research exploring the biology of stem cells and disease modeling. The educational hub is dedicated to training the scientific community in the basic biology of pluripotent stem cell research.

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Protocols

Find laboratory procedural methods that provides details on procedures, required equipments and instruments and includes information on troubleshooting and precautions. 

  • Immunostaining for Three Germ Layers with Antibodies against β-III-tubulin, AFP, and SMA
  • End Point RT- PCR to Confirm Absence of No Integration of Sendai Virus Vector into the iPSC Genome
  • iPSC Differentiation Into 3 Germ Layers by EB Formation
  • Differentiation of Neural Stem Cells (NSC) from Induced Pluripotent Stem Cells (iPSC)
  • Immunostaining of Induced Pluripotent Stem Cells (iPSC) for Pluripotency Markers Oct4, Sox2, Tra 1-60, and SSEA4

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Video Tutorial

Video tutorials by the Schuele Lab to provide educational guidance

  • Stem cell passaging
  • Skin Biopsy processing
  • Sterile Tissue Culture Techniques

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SCILL Posters

The Schuele laboratory has been a proud host institution for the San José State University Consortium for Stem Cell Internships in Laboratory-based Learning (SCILL) program. This program is supported by the Bridges to Stem Cell Research Grant from The California Institute of Regenerative Medicine (CIRM). In the Schuele laboratory, we provide students a 12-month, full-time internship in stem cell research to build their cell culture skills. At the end of the 12-month period, students presents posters to outline the projects that they were involved in. Students have also presented the posters that they derived at The Parkinson's Institute at scientific conferences.

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Other Resources

Additional resources with relevant training videos, protocols, and stem cell education. 

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